Detailed view for TGME49_293480

Basic information

TDR Targets ID: 257784
Toxoplasma gondii, MoeA N-terminal region (domain I and II) domain-containing protein
Source Database / ID:  ToxoDB 

pI: 7.8822 | Length (AA): 1255 | MW (Da): 133823 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00994   Probable molybdopterin binding domain
PF03453   MoeA N-terminal region (domain I and II)

Gene Ontology

Mouse over links to read term descriptions.
GO:0032324   molybdopterin cofactor biosynthetic process  
GO:0006777   Mo-molybdopterin cofactor biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
173 408 1g8l (A) 19 189 36.00 0.0000000011 0.75 0.04 1.47
988 1252 1uuy (A) 3 163 39.00 0 1 -0.01 0.65
189 873 1g8l (A) 36 408 33.00 0 1 -0.0690833 1.57
283 766 5erq (A) 380 678 36.00 0 0.99 -0.0181426 1.82
886 977 2ohd (A) 41 132 5.00 0.00073 0 0.132407 -0.25
996 1251 1jlj (A) 17 169 39.00 0.00000022 1 -0.0238159 0.37
1086 1201 1jlj (A) 58 174 22.00 0.00000000003 0.29 0.34553 -0.4
1215 1249 3pzy (A) 117 151 43.00 0.95 0.94 0.560088 -0.19

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile VEG Tachyzoite, ME49 Oocyst. Gregory Fritz HM
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.

Orthologs

Ortholog group members (OG5_127290)

Species Accession Gene Product
Arabidopsis thaliana AT5G20990   molybdenum cofactor biosynthesis enzyme CNX1
Brugia malayi Bm1_51550   Probable molybdopterin binding domain containing protein
Caenorhabditis elegans CELE_T06H11.4   Protein MOC-1
Dictyostelium discoideum DDB_G0287261   hypothetical protein
Drosophila melanogaster Dmel_CG2945   cinnamon
Escherichia coli b0827   molybdopterin molybdenumtransferase
Homo sapiens ENSG00000171723   gephyrin
Loa Loa (eye worm) LOAG_00059   hypothetical protein
Mycobacterium leprae ML0183   Probable molybdopterin biosynthesis protein MoeA1
Mus musculus ENSMUSG00000047454   gephyrin
Mycobacterium tuberculosis Rv0994   Probable molybdopterin biosynthesis protein MoeA1
Mycobacterium tuberculosis Rv0438c   Probable molybdopterin biosynthesis protein MoeA2
Mycobacterium ulcerans MUL_1390   molybdopterin biosynthesis protein MoeA2
Mycobacterium ulcerans MUL_4692   molybdopterin biosynthesis protein MoeA1
Neospora caninum NCLIV_000440   Molybdopterin biosynthesis protein, related
Oryza sativa 9272561   Os04g0661600
Onchocerca volvulus OVOC6881   Gephyrin homolog
Schistosoma mansoni Smp_067390   molybdopterin biosynthesis protein
Toxoplasma gondii TGME49_293480   MoeA N-terminal region (domain I and II) domain-containing protein

Essentiality

TGME49_293480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
b0827 Escherichia coli non-essential goodall
TGME49_293480 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_293480 (Toxoplasma gondii), MoeA N-terminal region (domain I and II) domain-containing protein
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